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Selective Pavlovian–to–Instrumental Transfer

Description: In selective PIT, there are two stages of training. In the Pavlovian stage, mice are placed in the operant chambers and are presented with two discriminative cues. In this stage, during each cue a specific reward is presented to the mouse, such that each cue becomes paired with that specific reward. In the instrumental phase, mice are placed in the operant chambers, and presented with one of the two levers. Responding on this lever, results in delivery of a specific reward (same rewards as used in Pavlovian stage). In a separate session, responding on a second lever results in delivery of second and different reward. Mice learn to associate each response with a specific reward. In the test stage, both levers are presented, and we asses the degree of responding to each lever during the cues. Typically, mice respond to a specific lever, during presentations of the cue associated with the same reward.

Purpose: This task assesses the impact of reward associated cues to induce a specific increase in motivational arousal resulting in increased specific instrumental responding. Targeted Neural System — BLA-Acc(shell)

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